Neon sites and data overview

This project utilizes the US National Ecological Observatory Network (NEON), which collects data about various ecological features and systems. The system that we are primarily interested in, Peromyscus mice and tick-borne disease, is covered by NEON’s small mammal trapping network.

Site locations

Our work focused primarily on eight sites shown by Figure 1 from 2022 to 2024. Information about the sites can be found on NEON’s Field Sites web page.

Upper northeast terrestrial NEON site locations used for this project.

Figure 1: Upper northeast terrestrial NEON site locations used for this project.

Small mammal data

Through their capture efforts at these sites, NEON has made 47,749 small mammal observations from 47 species and 25,071 unique individuals since its inception (Table 1). From 2022 to 2024, they made 11,958 observations of 6,700 individuals, 4,768 of which are either Peromyscus maniculatus or Peromyscus leucopus. NEON collects information about each individual mouse it catches. Some key traits used by this project are body size, sex, maturity, reproductive status (Table 2).


Table 1: Approximate counts of individuals1 from each species2 captured by NEON from its full duration and from our focal time window (2022 - 2024).
Species all time 2022-2024
Peromyscus leucopus 10034 3184
Peromyscus maniculatus 4722 980
Blarina brevicauda 3039 565
Myodes gapperi 1592 336
Peromyscus leucopus/maniculatus 1248 732
Napaeozapus insignis 1184 155
Tamias striatus 1119 206
Zapus hudsonius 601 54
Mus musculus 475 15
Microtus pennsylvanicus 454 49
Sorex cinereus 453 32
Glaucomys volans 445 198
Peromyscus sp. 242 51
Glaucomys sp. 190 10
Sigmodon hispidus 173 21
Mammalia sp. 151 147
Reithrodontomys humulis 93 80
Glaucomys sabrinus 70 19
Sorex arcticus 44 44
Sorex fumeus 39 11
Microtus pinetorum 28 2
Sorex sp. 24 4
Cryptotis parva 23 15
Tamiasciurus hudsonicus 18 3
Tamias minimus 15 15
Tamias minimus 15 1
Sylvilagus floridanus 10 2
Ochrotomys nuttalli 8 1
Synaptomys cooperi 7 1
Mustela frenata 4 4
Rodentia sp. 4 4
Condylura cristata 3 3
Rattus rattus 3 3
Didelphis virginiana 3 2
Blarina sp. 3 2
Mustela erminea 2 2
Cricetidae sp. 2 2
Mustela sp. 2 1
Sorex hoyi 2 2
Sorex palustris 2 2
Tamias sp. 2 1
Oryzomys palustris 2 2
Mustela nivalis 1 1
Sciurus carolinensis 1 1
Dipodidae sp. 1 1
Sorex longirostris 1 1
Sorex dispar 1 1


Table 2: Ten random small mammal observations subset. Columns correspond to observation ID, individual ID, capture data, life stage, sex, weight (g), tail length (mm), testes status (scrotal/nonscrotal), vagina condition (swollen or plugged).
obs ind date age sex weight tail testes vagina
53 7730 2024-09-26 adult M 24 86 scrotal NA
103 7696 2024-08-28 adult M 19 70 nonscrotal NA
125 7715 2024-08-30 subadult F 20 80 NA neither
132 6803 2022-04-25 adult F 32 86 NA not plugged
186 8034 2024-09-28 adult F 28 89 NA neither
259 8061 2024-05-08 adult F 26 76 NA neither
291 7843 2024-07-02 adult M 20 81 nonscrotal NA
319 8059 2024-05-08 adult F 28 82 NA plugged
353 7954 2023-07-12 adult M 21 69 nonscrotal NA
354 7051 2023-09-08 adult F 19 81 NA neither

Disease prevalence across sites

TODO: Borrelia prevalence in ticks…

Habitat differences across sites

TODO

Community differences across sites

  • small-mammal communitites: TODO

  • Other animal communities (e.g., deer, predators): TODO

Other data

In addition to the small mammal data product, we also use other NEON-collected data including

Further, our working group collected supplemental data in 2022-2024 including

  • Times at which individuals were captured, which is indicative of their activity/behavior;

  • Refined estimates of Borrelia infection, which are utilize a detection method that is more sensitive than those used by NEON, including quantitative pathogen burden; and

  • Expression of six key genes, thought to be important for resistance to and tolerance of Borrelia infection.

Much of this data will be explored in later sections.


  1. Individuals were sometimes labeled as multiple species across their capture record and double-counted by this table. The totals given in the text above are accurate.↩︎

  2. There are occasional misidentifications.↩︎